Journal: Journal of Cancer

Article Title: Ubiquitin specific peptidase 5 enhances STAT3 signaling and promotes migration and invasion in Pancreatic Cancer

doi: 10.7150/jca.48536

Figure Lengend Snippet: Correlation between USP5 expression and clinicopathological features of patients with pancreatic cancer

Article Snippet: Human pancreatic cancer cell lines (PL45, PSN1, MIAPaCa2, Panc1, HPAFII, panc03.27, BxPC3, Capan2, AsPC1, CFPAC1, Hs766T, SW1990, Capan1) and the normal pancreatic ductal epithelial cells (HPDE6.C7) were purchased from American Type Culture Collection.

Techniques: Expressing

Journal: Journal of Cancer

Article Title: Ubiquitin specific peptidase 5 enhances STAT3 signaling and promotes migration and invasion in Pancreatic Cancer

doi: 10.7150/jca.48536

Figure Lengend Snippet: Univariate and multivariate analysis of clinicopathological factors in patients with pancreatic cancer

Article Snippet: Human pancreatic cancer cell lines (PL45, PSN1, MIAPaCa2, Panc1, HPAFII, panc03.27, BxPC3, Capan2, AsPC1, CFPAC1, Hs766T, SW1990, Capan1) and the normal pancreatic ductal epithelial cells (HPDE6.C7) were purchased from American Type Culture Collection.

Techniques:

Journal: Journal of Cancer

Article Title: Ubiquitin specific peptidase 5 enhances STAT3 signaling and promotes migration and invasion in Pancreatic Cancer

doi: 10.7150/jca.48536

Figure Lengend Snippet: High USP5 expression suggests poor prognosis and shorter OS. (A) The overall survival curves for the high-USP5 expression group and the low-USP5 expression group; the difference is statistically significant ( P < 0.05). (B) USP5 expression levels in normal or pancreatic cancer tissues retrieved from GEPIA database. TPM, transcripts Per Million. (C) The overall survival curves for USP5 in ICGC database (ICGC-PACA-AU), P < 0.05.

Article Snippet: Human pancreatic cancer cell lines (PL45, PSN1, MIAPaCa2, Panc1, HPAFII, panc03.27, BxPC3, Capan2, AsPC1, CFPAC1, Hs766T, SW1990, Capan1) and the normal pancreatic ductal epithelial cells (HPDE6.C7) were purchased from American Type Culture Collection.

Techniques: Expressing

Journal: Journal of Cancer

Article Title: Ubiquitin specific peptidase 5 enhances STAT3 signaling and promotes migration and invasion in Pancreatic Cancer

doi: 10.7150/jca.48536

Figure Lengend Snippet: Increased USP5 expression suggests correlation with pancreatic cancer metastasis. (A) USP5 expression in lymph node and primary origin in paraffin-embedded tissues from pancreatic cancer patients.(magnification, 200×) (B) IHC score in metastasis lymph node (MLN) and primary origin. (C) The expression of USP5 examined by qPCR in eight cell lines derived from pancreatic cancer, five cell lines derived from metastasis and one normal pancreatic epithelial cell line. * P < 0.05; ** P < 0.01; *** P < 0.001.

Article Snippet: Human pancreatic cancer cell lines (PL45, PSN1, MIAPaCa2, Panc1, HPAFII, panc03.27, BxPC3, Capan2, AsPC1, CFPAC1, Hs766T, SW1990, Capan1) and the normal pancreatic ductal epithelial cells (HPDE6.C7) were purchased from American Type Culture Collection.

Techniques: Expressing, Derivative Assay

Journal: Journal of Cancer

Article Title: Ubiquitin specific peptidase 5 enhances STAT3 signaling and promotes migration and invasion in Pancreatic Cancer

doi: 10.7150/jca.48536

Figure Lengend Snippet: USP5 overexpression promotes the progression and metastasis of pancreatic cancer. (A) Overexpressed USP5 in Panc1 cells.(B) Overexpression of USP5 improved the viability of Panc1 cells. (C) Overexpression of USP5 enhanced colony formation of the Panc1 cells. (D-E) Transwell assays evaluated the effect of USP5 overexpression on migration and invasion of Panc1 cells after 24 hours. (F) Wound healing assay of Panc1 cells transfected with pENTER and USP5. Results were measured after 24 hours. (G) The expression level of EMT related markers in USP5 overexpression cells. Scale bar 100 µm * P <0.05, ** P <0.01, *** P < 0.001, Student's unpaired t-test.

Article Snippet: Human pancreatic cancer cell lines (PL45, PSN1, MIAPaCa2, Panc1, HPAFII, panc03.27, BxPC3, Capan2, AsPC1, CFPAC1, Hs766T, SW1990, Capan1) and the normal pancreatic ductal epithelial cells (HPDE6.C7) were purchased from American Type Culture Collection.

Techniques: Over Expression, Migration, Wound Healing Assay, Transfection, Expressing

Journal: Journal of Cancer

Article Title: Ubiquitin specific peptidase 5 enhances STAT3 signaling and promotes migration and invasion in Pancreatic Cancer

doi: 10.7150/jca.48536

Figure Lengend Snippet: USP5 knockdown inhibits the progression and metastasis of pancreatic cancer. (A) Knockdown USP5 in AsPC1 cells. (B) Knockdown USP5 inhibit the viability of AsPC1 cells.(C) Knockdown USP5 inhibit colony formation of the AsPC1 cells. (D-E) Transwell assays evaluated the effect of USP5 knockdown on migration and invasion in AsPC1 cells after 24 hours. (F) Wound healing assay of AsPC1 cells transfected with shUSP5. Results were measured after 24 hours. (G) The expression level of EMT related markers in USP5 knockdown cells. Scale bar 100μm * P <0.05, ** P <0.01, *** P < 0.001, Student's unpaired t-test.

Article Snippet: Human pancreatic cancer cell lines (PL45, PSN1, MIAPaCa2, Panc1, HPAFII, panc03.27, BxPC3, Capan2, AsPC1, CFPAC1, Hs766T, SW1990, Capan1) and the normal pancreatic ductal epithelial cells (HPDE6.C7) were purchased from American Type Culture Collection.

Techniques: Knockdown, Migration, Wound Healing Assay, Transfection, Expressing

Journal: Journal of Cancer

Article Title: Ubiquitin specific peptidase 5 enhances STAT3 signaling and promotes migration and invasion in Pancreatic Cancer

doi: 10.7150/jca.48536

Figure Lengend Snippet: USP5 was proved to mediate STAT3 signaling in pancreatic cancer cells. (A) Overexpress USP5 in Panc1 cells activate STAT3 signaling. (B) Knockdown USP5 in AsPC1 cells inhibit STAT3 signaling. Data were quantified by densitometric analysis with ImageJ software. * P < 0.05; ** P < 0.01; *** P < 0.001.

Article Snippet: Human pancreatic cancer cell lines (PL45, PSN1, MIAPaCa2, Panc1, HPAFII, panc03.27, BxPC3, Capan2, AsPC1, CFPAC1, Hs766T, SW1990, Capan1) and the normal pancreatic ductal epithelial cells (HPDE6.C7) were purchased from American Type Culture Collection.

Techniques: Knockdown, Software

Journal: NPJ Precision Oncology

Article Title: Connectivity mapping-based identification of pharmacological inhibitor targeting HDAC6 in aggressive pancreatic ductal adenocarcinoma

doi: 10.1038/s41698-024-00562-5

Figure Lengend Snippet: Connectivity mapping was used to identify negatively connected drugs specific to PC datasets. a Schematic representation of overall study design was followed to identify and validate specific therapeutic for PC. b The bar graph represents a number of tumors and normal samples within the four microarray datasets GSE18670 (24 samples), GSE32676 (32 samples), GSE15471 (78 samples), and GSE16515 (52 samples). A differential gene expression was carried out using the limma package from R bioconductor to identify top differentially expressed genes between normal and tumor samples. c Venn diagrams represent negatively connected drugs across the four datasets. Nine common drugs were identified as being common between all the datasets. d Heat maps represent the connectivity scores of all 9 commonly negatively connected drugs. e Venn diagram represents a comparison of highly specific drugs identified for PC with PC cell lines, human PC tumoroids, human tissue, and patient-derived xenografts (PDX) datasets. The negatively connected drugs were compared to the nine drugs identified from the human tissue samples. ISOX was identified as the only drug common between all these four models. f The structure of ISOX (CAY 10603) as obtained for CMAP shows a clear HDACi moiety. ISOX target HDAC 6 is highly expressed in PC cells and tissues. g Immunoblot of HDAC 6 in a panel of normal immortalized pancreatic cells and PC cell lines with β-actin as an internal loading control. h , i Whipple surgery resected PC tissues were stained with HDAC 6 specific antibody and counterstained with hematoxylin and eosin stains. Representative images were taken using the objective lens of 20× and eyepieces with 10× magnifications. Total magnification of the images is 200× (objective lens (20×) × eyepieces (10×)). Data represented as Dot and violin plot demonstrating the protein expression level of HDAC 6 in near-by normal, PanINs, well, moderately, and poorly differentiated stages of PC (in whole tissue). h Linear mixed model show HDAC 6 composite score is significant ( p = 0.013) between two groups (moderate and normal adjacent to tumor, p = 0.011). Representative images of immunohistochemical staining of HDAC 6 in human Whipple surgery resected PC samples with varied adjacent normal, PanINs, and various differentiated stages of PC (Magnification in 200×).

Article Snippet: Human pancreatic cell lines (AsPC1, MiaPaCa2, CD18/HPAF, and CFPAC1) were obtained from ATCC and routinely confirmed and authenticated using short tandem repeat (STR) profiling during the experiments.

Techniques: Microarray, Gene Expression, Comparison, Derivative Assay, Western Blot, Control, Staining, Expressing, Immunohistochemical staining